Cell 188:6845-6860

Cryo-EM analysis of coronin 2 with actin and cofilin.

Cell Death and Disease 16:690 ;

In this work BioID analysis identified coronin 2 as a cascade 3 interactor and that reducing cascade 3 expression lowers coronin 2 phosphorylation.

J. Cell Biol. 224:e202407014

Here, coronin 2 was identified as a substrate of the deubiquitinase USP45.

Molecules (2024), 29, 4818

In this study several coronin proteins (cor1-3 and 5,6) were detected by proteomics of isolated endocytic vesicles.

Biomedicine & Pharmacoth (2023) 161:114449

Qi et al. report on complexes between coronin 2, Inositol hexakisphosphate kinase 1 (IP6K1), Arp2/3 and beads containing inositol phosphate moieties.

J Cell Biol (2022) 221:e202111126

Generation and characterisation of a cor2/3 double knockout cell line. Data are shown that contrary to previous observations (e.g., Cai et al 2007, PMID: 17350576) excessive cofilin accumulates in coronin null lamellipodia.

Curr Biol (2021) 31:696-706.e9

Bio-ID analysis defines coronin 2 as an interactor of Smoothelin-like 2 protein, and coronin 2-GFP is shown to colocalize and interact with Smoothelin-like 2 protein.

Biomed Res (2021) 42:95-102

Here, it is shown that overexpression of TBC1D8B, a GTPase-activating protein, in colon carcinoma cells (HCT116) leads to increased coronin 2 protein levels.

Stem Cell Res Ther (2020) 11:79

Human plasma and platelet/horse serum-based culturing of bone marrow-derived mesenchymal stromal cells are shown to increases expression of coronin 2.

Animals (2020) 10:880

Fibroblasts cultured in different % of serum show different levels of coronin 2 expression.

Biochem. Biophys. Res. Comm. (2020) 526:999-1004

In this study, it is shown that depletion of coronin 2 by siRNA decreases TNFα-induced apoptosis and that coronin 2 binds to Fa-associated death domain protein (FADD).

Front Cell Dev Biol (2020) 8:708

In this paper it is reported that in endothelial cells coronin 2 localises at cell-cell junctions. Furthermore, coronin 2 was found to interact with integrin-linked kinase and that coronin 2 depletion affects the formation of Junction-associated intermitted lamellipodia (JAIL).

Platelets (2019) 31:913-924

This paper reports abundant expression of coronin 1, 2 and 3 in platelets, where they assemble with each other and with GαS. Furthermore, it is shown that platelet stimulation does not affect their subcellular localization.

Cell Res (2019) 29:432-445

In this work, it is shown that coronin 2 silencing modulates S-phase human mammary epithelial cells.

Nat Struct Mol Biol (2018) 25:528-537

Here, TIRF microscopy is used to analyse in vitro binding of recombinantly-expressed SNAP-tagged coronin 2 to F-actin in the presence of different nucleotides.

Biomed Pharmacother (2017) 95:235-244

A number of active compounds from Moutan Cortex (MC), a well-known Chinese medicine for promoting blood circulation and relieving blood stasis, were found to upregulate coronin 2 expression.

Hippocampus (2017) 27:845-859

Here, proteomic analysis of amnesic versus control hippocampi in mice shows increase in coronin 2 protein levels.

Biochem. Biophys. Res. Comm. (2017) 490:574-579.

Kim et al. show data interaction of coronin 2 with tumour growth factor receptor β1 and that its signalling is negatively affected by coronin 2.

Cell Sign (2016)28:1817-1825

These authors report that in HUVECs coronin 2 depletion increases cell motility and that coronin 2 is phosphorylated by p38α mitogen-activated protein kinase (MAPK).

Cell Cycle (2016) 15:3033-3041

In this study, it is shown that coronin 2 depletion in MCF-7 cells, a human mammary epithelial cell line, results in a loss of non-muscle myosin II (NMII) motor proteins at adherent junctions.

Dev Cell (2016) 37:58-71

Here, depletion of coronin 2 in Caco-2 cells using RNAi was shown to reduce the levels of junctional E-cadherin and affect re-assembly of F-actin following Latrunculin A treatment.

Curr Biol (2016) 26:1312-8

In this work it is reported that coronin 2 knock down in SV40-immortalized mouse embryonic fibroblasts results in a reduction of tropomyosin isoform Tpm1.8/9 enrichment in lamellipodia.

J Cell Biol (2016) 214:197-213

This study analyses the role of actin in exosome release, and reports that upon Rab27a and cortactin knock down in cells there is an increase of coronin 2 localization at invadopodia.

Nat Cell Biol (2016) 18:76-86

Silencing of coronin 2/3 increases actin tail lengths in infected HeLa cells.

J Mol. Biol. (2016) 428:1604-16

This study reports on the in vitro actin dynamics as modulated by actin depolymerising factor (ADF)/cofilin proteins (using recombinantly expressed His/Snap-tagged coronin 2 constructs as described in Janssens..Goode 2015, PMID: 25995115).

Nat Commun (2015) 6:7202

In this study, recombinantly expressed His/Snap-tagged coronin 2 is used to analyze actin dynamics in vitro.

Biochem. Biophys. Acta (2015) 1853:409-21

A protein-protein interaction screen of TRIM15 with the integrin adhesome identified several TRIM15 interacting proteins including coronin 2.

J Cell Biol (2015) 208:961-74

In this paper data are shown on the Waf1 Cip1 stabilizing protein 39 (WISp39), an interaction of HSP90, and its interaction with coronin 2.

Mol Cell Biol (2014) 34:3388-406

Following up on Castro-Castro et al (2011, PMID: 21873980), Ojeda et al report in this paper that coronin 2 (and 1) through F-actin modulation and myosin II inactivation induce Rac1 translocation to the plasma membrane.

Exp Cell Res (2013) 319:1365-81

In this paper, it is reported that coronin 2 is detected in mouse embryos in the developing heart and that coronin 2 expression can be modulated by Wnt ligands, and that silencing coronin 2 results in reduced migration in NIH3T3 cells.

J Biol Chem (2013) 288:33324-34

This paper addresses the results of methamphetamine addition on the phosphorylation of serine-2 on coronin 2 via MAPK signalling. See also the work by Cai..Bear’05, PMID: 16027158) of protein kinase C and ser-2 phophorylation.

PloS One (2013) 8:e63007

In this work it is shown that sphingosine-1-phosphate stimulates coronin 2 localisation to lamellipodia and that coronin 2 mRNA silencing attenuates this process.

Anal. Chem. (2012) 84:10614−10620

These authors describe the generation of coronin 2-depleted cells (through shRNA) using microfabricated arrays.

J Biol Chem (2012) 287:21836–21845

In this paper, the authors shows complex formation of coronin 1/2 with ROCK2.

Circ Res. (2012) 111:56-65

This paper shows that coronin 2 depletion in cells increases motility.

Brain (2011) 134:2134–2148

Overexpression of histone acetyltransferase p300 promotes axonal regeneration and occupies and acetylates the promoters of the coronin 2-encoding gene as well as the genes encoding the growth-associated protein-43 and Sprr1.

Mol. Psychiatry (2011) 16:1105–1116

This paper reports that depletion of dysbindin, a gene associated with schizophrenia, mimics p53 depletion (see Di Giovanni..Faden 2006, PMID: 16946709) in terms of coronin 2 downregulation.

J. Exp. Med. (2011) 208:1777-1787

In this work, a mouse mutant lacking coronin 2 is reported that has no obvious phenotype. The authors also report that a coronin 1/2 double knock out mouse shows enhanced mast cell degranulation.

J. Biol. Chem. (2008) 283:32542–32552,

This paper includes an analysis of the enzyme activity of the slingshot phosphatase on coronin 2 and reports coronin 2 to not be no dephosphorylated by slingshot, in apparent contrast the results shown bye Cai..Bear, 2007 (PMID: 17350576).

Phytochem. Lett. (2008) 1:135–138

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In this study it was found that coronin 2 is upregulated following salvianolic acid stimulation of platelets.

Cell (2008) 34:828–842

Analysis of the effect of coronin 2 on actin branching/debranching in vitro and analysis of coronin 2-EGFP localisation in transfected cells.

Cell (2007) 128:915–929

Analysis of the role for coronin 2 in cofilin activity; Coronin 2 is shown to be dephosphorylated by slingshot, results that are in apparent contrast to the work by Kurita et al, 2008 (PMID: 18809681). Further, whereas here cofilin is shown to be reduced upon coronin 2 depletion, subsequent work shows cofilin to be increased following coronin 2 deletion (see King et al., PMID: 35657370).

J. Cell Sci. (2007) 120:2796-2806

Here it is shown that ectopic expression of GFP-coronin 2 induces actin cables in Drosophila S2 cells and that using in vitro sedimentation analysis Arg 30 is required for F-actin-binding. Furthermore, the authors report that they fail to obtain evidence for coronin 1/2-mediated cofilin binding to F-actin, and that coronin 2 inhibits cofilin-induced disassembly, in apparent contrast to the results from Brieher et al (2006) PMID: 17060499.

EMBO J. (2006) 25:4084–4096

Here, data are shown that coronin 2 is a transcriptional target of P53 and that overexpression of GFP-tagged coronin 2 rescues neurite outgrowth in PC12 cells expressing dominant negative p53.

J. Biol. Chem. (2005) 280:2084–2091

In this work it is shown that coronin 2 (and Rab13) expression is induced upon spinal injury and that siRNA against coronin 2 reduces neurone outgrowth while over expression of cororonin 2 induces neuronal outgrowth.

J. Biol. Chem. (2005) 280:31913–31923

This paper reports that GFP or Myc-tagged coronin 2 exclusively homodimerizes without complexing with other coronins, and that coronin 2 can be co-immunoprecipitated with p34-Arc in fibroblasts cell lines through protein kinase C-mediated phosphorylation.

J. of Repr. Dev. (2003) 49:547–552

Neonatal rat hippocampi form male, as well as upon estradiol-treatment show enhanced coronin 2 expression levels.

DNA and Cell Biol (1998) 17:779-787

Okomura et al. describe the cloning of a set of 4 homologous proteins with homology to Dictyostelium coronin, and designate these coronin 1, 2, 3 and 4.