J. Biol. Chem. (2025) 301(3) 108191

Here, in vitro assembly of E. coli-expressed Crn1 constructs are analysed for interaction.

Cell Reports 42, 112594

Here, a number of Crn1 constructs fused to mRuby, Myc and HIS tags are analyzed for oligomerisation and in vitro F-actin binding and crosslinking .

Cells 2023 12(3):484.

Addition of cadmium selenide zinc sulfide quantum dots to yeast increases Crn1-GFP expression.

Int. J. Mol. Sci. (2020) 21:1469

Sedimentation analysis of Crn1 with rabbit actin in vitro

Genetics 2019 213(3):819-834

Mutations in Crn1 revert the recombinatorial repair deficiencies upon deletion of the chromatin remodelling complex factor swr1 in some (BY4741), but not other (W303) yeast strains.

J Mol Biol. 2017 429(2):237-24

Sokolova and colleagues use single-particle electron microscopy and recombinantly expressed and purified proteins to compare the structures of Arp2/3 complex bound to three different inhibitory ligands: glia maturation factor (GMF), GST-Crn1, and arpin.

Cytoskeleton 2015 Jul;72(7):349-61

Here, Ydenberg et al. generate a series of single, double and triple mutants including the crn1-null strain, and show that while the single mutant does not show a phenotype multiple mutants are affected in endocytosis and growth.

BBRC 2015 Feb 27;458(1):208-13.

In this paper Crn1 is proposed to be involved in septic assembly and cell cycle regulation.

J Mol Biol (2015) 5;427(19):3137-47

Mikati et al. use TIRF using recombinantly expressed and purified proteins to analyse actin conformation in the presence and absence of Crn1/cofilin.

Nat Struct Molo Biol 2014 21:1075-81

Ge et al. report the cryo-EM structures for actin filaments in complex with the β-propeller domain of Crn1.

J Biol Chem 286(19):17039-46

Liu et al describe Crn1 mutants that affect Arp2/3 activity in vitro and result in defective actin patched in cells. See also the papers by Lin..Cooper 2010, Heil-Chapdelaine..Cooper 1998, Goode..Barnes 1999, Sagot..Daignan-Fornier 2006, reporting a normal actin cytoskeleton upon Crn1 deletion.

J Biol Chem 285(45):34899-908

Here, Ghandi et al. report on the in vitro interaction of Crn1 mutants with actin.

J Cell Sci 123(Pt 8):1329-42

Lin et al. analyze actin-mediated processes with and without Crn1, and conclude that there is virtually no phenotype upon loss of Crn1 in actin-mediated processes, nor inhibition of cofilin recruitment to actin patches (see also the study by Ghandi et al. (2009, PMID: 19450534). Furthermore, they address the in vivo relevance of the bursting activity described for cofilin, coronin and Aip1 in the turnover of actin patches (Kueh et al. (2008, PMID: 18663144).

Mol. Biol. Cell 21:2894-2904

GFP-tagged Crn1, among several other fluorescently-tagged proteins (actin, Arp2/3, capping protein, fimbrin) is analysed by live imaging during endocytosis.

Eukaryot Cell 2010, 9(11):1788-97

Here, Crn1 was localised at the growing tip of S. pombe while forming multicellular structures.

J Cell Biol 185(6):1097-110

Burtson et al. describe a screen for endocytosis regulators and include Crn1 as one of the hits.

Mol Cell 34(3):364-74

Gandhi et al. report that the role for Crn1 in in vitro actin disassembly through cofilin modulation depends on the nucleotide state of actin.

Plos Biol Jan;6(1):e1 doi: 10.1371/journal.pbio.0060001

Galetta et al. investigate a potential role for Crn1 in the dynamics of actin patches, an Arp2/3-dependent process, and their results suggest a minor delay in patch formation when Crn1 is absent.

Mol Biol Cell 17(11):4645-55

This paper analyses the yeast quiescent state, with respect to actin that is organised in ‘actin bodies’ and report that in strains lacking Crn1 there is no alteration of actin bodies.

Nat Struct Mol Biol 12(1):26-31

Rodal et al. analyze Arp2/3 conformations with and without recombinantly (in E. coli) expressed Crn1.

J Cell Biol 159(6):993-1004

Humpries et al. show co-IP of Arp2/3 with Crn1 and co-migration of Crn1 and Arp2/3 when yeast lysates are analysed by sucrose gradients and that the Crn1 coiled coil region inhibits Arp2/3-mediated actin nucleation in vitro.

J Cell Biol 144(1):83-98

Here, the authors report on in vitro assembly of recombinantly (in E. coli) expressed Crn1 with purified tubulin and actin and show that deletion of Crn1 results in a normal actin-based cytoskeleton and normal yeast growth.

Curr Biol 8(23):1281-4

In this first report on Crn1 in S. cerevisiae, the authors conclude that they fail to find defects in actin- and microtubule-based processes in the absence of Crn1.